Wayne State University

Douglas Ruden, Ph.D.

Associate Professor, Director of Epigenomics
IEHS and OB/GYN
(313) 577-6688 (Phone)
(313) 577-0082 (Fax)
douglasr@wayne.edu

Profile Picture for Douglas Ruden
Research Interests

Toxicogenomics of heavy metal exposure using Drosophila and human embryonic stem cell models.

Selected Publications

1. Topol J, Ruden DM, Parker CS. Sequences required for in vitro transcriptional activation of a Drosophila hsp 70 gene. Cell 1985; 42:527-537. (PMID: 4028160)

2. Ruden DM, Ma J, Li Y, Wood K, and Ptashne M. Generating yeast transcriptional activators containing no yeast protein sequences. Nature 1991; 350(6315):250-252. (PMID: 2005981)

3. Sollars V., Lu X, Xiao L, Wang X, Garfinkel MD, Ruden DM. Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. Nat Genet 2003:33:70-74. (PMID: 12483213) This was a cover story and was accompanied by a News and Views: Rutherford, S. and Henikoff, S. (2003). Quantitative epigenetics, Nat Genet 33, 6-8. See also Nature Reviews Genetics article on this paper, Baxter, C. (2003) Now you see it �now you don�t! Nat. Rev. Genet. 4, 81.

4. Hirsch, HVB., Mercer J, Sambaziotis H, Huber M, Stark DT, Torno-Morley T, Hollocher K, Ghiradella H, Ruden DM. Behavioral effects of chronic exposure to low levels of lead In Drosophila melanogaster. Neurotoxicology 2003; 24:435-442. (PMID: 12782108)

5. Garfinkel MD, Sollars V, Lu X, and Ruden DM. Multigenerational selection and detection of altered histone acetylation and methylation patterns: toward a quantitative epigenetics in Drosophila. In Epigenetics Methods, editor T. Tollefsbol, Methods Mol Biol 2004;287:151-168. (PMID: 15273410)

6. Ruden DM, Xiao L, Garfinkel MD, Lu X. Hsp90 and Environmental Impacts on Epigenetic States � A Model for the Trans-Generational Effects of Diethylstilbestrol (DES) on Uterine Development and Cancer in the Granddaughters of Exposed Pregnant Females. Hum Mol Genet 2005; 14: R149-R55. (PMID: 15809267)

7. Page GP, Ruden DM. Combining high dimensional biological data to study complex diseases and quantitative traits. In DNA Microarrays and Related Genomics Techniques � Design, Analysis, and Interpretation of Experiments, Edited by D. B. Allison, G. P. Page, T. M. Beasley, and J. W. Edwards, Published by Chapman & Hall/CRC, Boca Raton, FL 2006, pages 335-360. (Book; PMID: not available)

8. Ruden, D. M. (2007). Personalized medicine and quantitative trait transcripts. Nat Genet. 39(2), 144-145. (PMID: 17262025)

9. Hirsch, H.V.B., Possidente, D., Averill, S., Despain, T.P., Buytkins, J., Thomas, V., Goebel, W.P., Shipp-Hilts, A., Wilson, D., Hollocher, K., Possidente, B., Lnenicka, G., and Ruden, D.M. (2009). Variations at quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster. Neurotoxicology 30(2), 305-311. (PMID: 19428504)

10. Levin ED, Aschner M, Heberlein U, Ruden D, Welsh-Bohmer KA, Bartlett S, Berger K, Chen L, Corl AB, Eddins D, French R, Hayden KM, Helmcke K, Hirsch HV, Linney E, Lnenicka G, Page GP, Possidente D, Possidente B, Kirshner A. (2009). Genetic Aspects of Behavioral Neurotoxicology 30 (5), 741-753. (PMID: 19647018)

11. Ruden, D.M., Chen, Possidente, L.D., Possidente, B., Rasouli, P., Wang, L., Lu, X., Garfinkel, M.D., Hirsch, H.V., Page, G. (2009). Genetical toxicogenomics in Drosophila identifies master-modulatory loci that are regulated by developmental exposure to lead, Neurotoxicology 30(6), 898-914. (PMID: 19737576)

12. He, T., Hirsch, H. V., Ruden, D. M., and Lnenicka, G. A. (2009). Chronic lead exposure alters presynaptic calcium regulation and synaptic facilitation in Drosophila larvae. Neurotoxicology 30 (5), 777-784. (PMID: 19732793)

13. Ruden, D.M., Possidente, D., Lnenicka, G., and Hirsch, H.V.B. (2010). Gene-Environment Interactions: Drosophila as a model for Toxicogenomics of Lead. The Encyclopedia of Environmental Health 29, 1-25. (PMID: none).

14. Elobeid, M.A., Padilla, M.A., Brock, D.W., Ruden, D.M., and Allison, D.B. (2010) Putative Endocrine Disrupting Chemicals and Obesity: An Examination of Dioxins in the NHANES 1999-2002 Data. Intern. J. Env. Res. Publ. Health 7(7), 2988-3005. (PMID: 20717554)

15. Padilla, M.A., Elobeid, M.A., Ruden, D.M., and Allison, D.B. (2010) An Examination of the Association of Selected Toxic Metals with Total and Central Obesity Indices: NHANES 99-02. Intern. J. Env. Res. Publ. Health, in press (PMID: 20948927).

16. Ruden, D.M. (2011). The (new) new synthesis and epigenetic capacitors of morphological evolution. Nat Genet 3(2), 88-89 (PMID: 21270834).

17. Nguyen, T.T., Shi, W. and Ruden, D. M. (2011). CloudAligner: A fast and full-featured MapReduce based tool for sequence mapping. BMC Research Notes 4(1), 171-181. (PMID: 21645377)

18. Cingolani, P., Platts P., Wang, L.L., Coon, M., Nguyen, T.T., Wang, L., Land, S.J., Ruden, D. M., and Lu, X. (2012). A Program for Annotating and Predicting the Effects of Single Nucleotide Polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly (Austin) 6(2), 80-92. (PMID: 22728672)

19. Cingolani, P., Patel, V.M., Coon, M., Nguyen, T.T., Land, S.J., Ruden, D. M., and Lu, X. (2012). Using Drosophila melanogaster as a model for genotoxic chemical mutational studies with a new program, SnpSift. Frontiers in Genetics 3:35. Epub 2012 Mar 15. (PMID: 22435069)

20. Niu, Q., Wang, W., Li, Y., Ruden, D.M., Wang, F., Li, Y., Wang, F., Song, J., Zheng, K. (2012). Low molecular weight heparin ablates lung cancer cisplatin-resistance by inducing proteasome-mediated ABCG2 protein degradation. PLoS One. 7(7):e41035. Epub 2012 Jul 23. (PMID: 22844424)

21. Senut. M.C., Cingolani, P., Sen, A., Kruger, A., Shaik, A., Hirsch, H., Suhr, S.T., Ruden, D. (2012). Epigenetics of early-life lead exposure and effects on brain development. Epigenomics 4(6), 665-674. (PMID: 23244311)

22. Hirsch, H.V., Lnenicka, G., Possidente, D., Possidente, B., Garfinkel, M.D., Wang, L., Lu, X., Ruden, D.M. (2012). Drosophila melanogaster as a model for lead neurotoxicology and toxicogenomics research. Front. Genet. 3, 68-78. (PMID: 22586431)

23. Schrey, A.W., Richards, C.L., Meller, V., Sollars, V., Ruden, D.M. (2012). The role of epigenetics in evolution: the extended synthesis. Genet Res Intl, Epub 2012. (PMID: 22567381).

24. Figueroa-Romero, C., Hur, J., Bender, D.E., Cataldo, M.D., Lunn, S., Pacut, C., Smith, A.L., Delaney, C.E., Yung, R., Ruden, D.M., Callaghan, B.C., Feldman, E.L. (2012). DNA methylation and gene expression in sporadic amyotrophic lateral sclerosis. PLoS One, in press.